The most critical parameter of the calibration is the Forward Efficiency Feff. This parameter is a function of frequency (antenna surface accuracy), but also of the receiver used (illumination) and of the Focus with a tendency to be too low if you are defocussed. If Feff is underestimated, Tsky is underestimated (See equation 8 and note that Tcab is always greater than Temi except in very bad sites...) and you may obtain anomalously low Water vapor content, and vice-versa.
The sideband gain ratio
is also a critical parameter.
is not only a scaling factor as obvious in Eq. 8, but
is also involved in the derivation of the atmospheric model since
the contributions from the atmosphere in image and signal bands are
considered. This effect will be important only if the opacities in both bands
are significantly different, as for the J=1-0 line of CO.
On the 30-m telescope, because of the design of the cold load, Tcold is also a function of frequency and receiver (it varies from 87 K at 230 GHz to 116 K at 75 GHz). The values of Tcold are fairly well known by the receiver engineers : ask them...